Cdh1^tm3Kem
Targeted Allele Detail

Summary

• Symbol: Cdh1^tm3Kem
• Name: cadherin 1; targeted mutation 3, Rolf Kemler
• MGI ID: MGI:3574476
• Synonyms: Ecad-In2floxFRT
• Gene: Cdh1 Location: Chr8:107329983-107396878 bp, + strand Genetic Position: Chr8, 53.18 cM, cytoband D
• Alliance: Cdh1^tm3Kem page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96963
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a Betageo cassette into the ATG codon a loxP site at the 5' end of exon 2 to generate Cdh1^tm6Kem. A second targeting vector inserted a PGK-hygromycin cassette flanked by FRT sites and a single loxP site at the 3' end of intron 2, thereby flanking intron 2 with loxP sites. (J:96963)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdh1 Mutation: 173 strains or lines available

References

• Original: J:96963 Stemmler MP, et al., E-cadherin intron 2 contains cis-regulatory elements essential for gene expression. Development. 2005 Mar;132(5):965-76
• All: 1 reference(s)