Zic5^tm1Sia
Targeted Allele Detail

Summary

• Symbol: Zic5^tm1Sia
• Name: zinc finger protein of the cerebellum 5; targeted mutation 1, Shinichi Aizawa
• MGI ID: MGI:3574814
• Synonyms: Opr^-
• Gene: Zic5 Location: Chr14:122696572-122703127 bp, - strand Genetic Position: Chr14, 65.96 cM, cytoband E5
• Alliance: Zic5^tm1Sia page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97076
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A sequence 172 bp upstream of the zinc finger motif at exon 1 was replaced with a cassette containing stop-IRES-lacZ-loxP-neo-loxP. RT-PCR of mutant embryos confirmed absence of transcript. Crossing with EIIa-cre mice removed the neo. (J:97076)

Expression

In Mice Carrying this Mutation:	30 assay results
In Structures Affected by this Mutation:	16 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Zic5 Mutation: 37 strains or lines available

References

• Original: J:97076 Furushima K, et al., Characterization of Opr deficiency in mouse brain: Subtle defects in dorsomedial telencephalon and medioventral forebrain. Dev Dyn. 2005 Apr;232(4):1056-61
• All: 2 reference(s)