Tpm3^tm2Pgun
Targeted Allele Detail

Summary

• Symbol: Tpm3^tm2Pgun
• Name: tropomyosin 3, gamma; targeted mutation 2, Peter Gunning
• MGI ID: MGI:3574955
• Gene: Tpm3 Location: Chr3:89979958-90008209 bp, + strand Genetic Position: Chr3, 39.2 cM
• Alliance: Tpm3^tm2Pgun page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97192
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance flanked by loxP sites was inserted into the gene, resulting in the deletion of the entire 9c coding and the upstream UTR and splice sites. Western blot confirmed absence of protein from this isoform in mutant brains. (J:97192)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tpm3 Mutation: 48 strains or lines available

References

• Original: J:97192 Vrhovski B, et al., Modification of the tropomyosin isoform composition of actin filaments in the brain by deletion of an alternatively spliced exon. Neuropharmacology. 2004 Oct;47(5):684-93
• All: 2 reference(s)