Tnfrsf4^tm1Mfb
Targeted Allele Detail

Summary

• Symbol: Tnfrsf4^tm1Mfb
• Name: tumor necrosis factor receptor superfamily, member 4; targeted mutation 1, Martin F Bachmann
• MGI ID: MGI:3575088
• Synonyms: OX40^-
• Gene: Tnfrsf4 Location: Chr4:156098300-156101069 bp, + strand Genetic Position: Chr4, 87.68 cM
• Alliance: Tnfrsf4^tm1Mfb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59204
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was created to introduce a stop codon at amino acid 150. A neomycin resistance cassette was also included in the vector for selection purposes. Absence of transcript was confirmed by RT-PCR of total RNA from mutants. Flow cytometry further showed absence of protein on the surface of stimulated mutant splenocytes. (J:59204)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnfrsf4 Mutation: 36 strains or lines available

References

• Original: J:59204 Kopf M, et al., OX40-deficient mice are defective in Th cell proliferation but are competent in generating B cell and CTL Responses after virus infection. Immunity. 1999 Dec;11(6):699-708
• All: 11 reference(s)