Ryr3^tm1Jms
Targeted Allele Detail

Summary

• Symbol: Ryr3^tm1Jms
• Name: ryanodine receptor 3; targeted mutation 1, Institute of Medical Science, Japan
• MGI ID: MGI:3575753
• Gene: Ryr3 Location: Chr2:112461700-113047441 bp, - strand Genetic Position: Chr2, 57.2 cM, cytoband E5-F3
• Alliance: Ryr3^tm1Jms page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58558
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Targeting replaced a 0.85-kb segment of the gene containing parts of exons 6 and 7 with a 1.8-kb Pgk-neo cassette having stop codons in all three reading frames and a polyadenylation signal. Immunoblot analysis of microsomal fractions of the cerebra, including hippocampi, of heterozygous and homozygous mice demonstrated reduction and absence, respectively, of detectable RYR3 versus those of wild-type mice. Mice of all three genotypes expressed similar total levels of RYR proteins in their cerebra. (J:58558)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ryr3 Mutation: 261 strains or lines available

References

• Original: J:58558 Futatsugi A, et al., Facilitation of NMDAR-independent LTP and spatial learning in mutant mice lacking ryanodine receptor type 3. Neuron. 1999 Nov;24(3):701-13
• All: 2 reference(s)