Grid2^tm2Mim
Targeted Allele Detail

Summary

• Symbol: Grid2^tm2Mim
• Name: glutamate receptor, ionotropic, delta 2; targeted mutation 2, Masayoshi Mishina
• MGI ID: MGI:3576122
• Synonyms: GluRdelta2^flox
• Gene: Grid2 Location: Chr6:63232860-64681307 bp, + strand Genetic Position: Chr6, 29.77 cM
• Alliance: Grid2^tm2Mim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97405
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP sequence and an Flp-flanked neo was inserted 381 bp upstream of exon 12 and a second loxP sequence was inserted downstream of exon 12. Exon 12 encodes the putative transmembrane segment M3. Crossing with Tg(EIF1A-flp)66Mim removed the neo. Western blot analysis demonstrated that protein expression in cerebellar homogenates from mutants was comparable to that of wild-type. (J:97405)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grid2 Mutation: 85 strains or lines available

References

• Original: J:97405 Takeuchi T, et al., Control of synaptic connection by glutamate receptor delta2 in the adult cerebellum. J Neurosci. 2005 Feb 23;25(8):2146-56
• All: 3 reference(s)