B3gnt2^{Gt(KST308)Byg}
Gene trapped Allele Detail

Summary

• Symbol: B3gnt2^{Gt(KST308)Byg}
• Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2; gene trap KST308, BayGenomics
• MGI ID: MGI:3576265
• Synonyms: B3gnt2^{Gt1(Betageo-ALPP)Gas}, Beta3GnT1^-
• Gene: B3gnt2 Location: Chr11:22784739-22810336 bp, - strand Genetic Position: Chr11, 14.22 cM
• Alliance: B3gnt2^{Gt(KST308)Byg} page

Mutation origin

• Mutant Cell Line: KST308
• Germline Transmission: Earliest citation of germline transmission: J:97372
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1TMpfs Vector Type: secretory trap
• Mutation details: A vector encoding Betageo and human ALPP integrated into exon 2 at position 686 of the coding reigon and truncated the protein after 228 amino acids, removing the "DxD" and other conserved C-terminal motifs required for protein activity. (J:97372)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  KST308	CC144696 ; (MGI Seq Detail)	5' RACE	Chr11:22786500-22786769 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  1 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	32 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any B3gnt2 Mutation: 156 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:97372 Henion TR, et al., Beta1,3-N-acetylglucosaminyltransferase 1 glycosylation is required for axon pathfinding by olfactory sensory neurons. J Neurosci. 2005 Feb 23;25(8):1894-903
• All: 7 reference(s)