Sema3b^tm1.2Brd
Targeted Allele Detail

Summary

• Symbol: Sema3b^tm1.2Brd
• Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B; targeted mutation 1.2, Allan Bradley
• MGI ID: MGI:3576464
• Synonyms: m2, Sema3B^Brdm2
• Gene: Sema3b Location: Chr9:107474873-107486428 bp, - strand Genetic Position: Chr9, 58.31 cM
• Alliance: Sema3b^tm1.2Brd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97316
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exons 1-6, encoding the first 163 amino acids and ~40% of the SEMA domain, were flanked with loxP sites. An FRT-flanked puromycin resistance cassette was inserted into intron 6. ES cells were transfected with Cre to remove the floxed sequence containine exons 1-6. In situ hybridization of mutant embryos demonstrated lack of expression in rib primordial and regions of the head. RT-PCR showed transcripts from exons 7 and higher, though the transcripts do not have the full-length SEMA domain and therefore result in a functionally null protein. (J:97316)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sema3b Mutation: 31 strains or lines available

References

• Original: J:97316 van der Weyden L, et al., Null and conditional semaphorin 3B alleles using a flexible puroDeltatk loxP/FRT vector. Genesis. 2005 Apr;41(4):171-8
• All: 1 reference(s)