Dicer1^tm1Gz
Targeted Allele Detail

Summary

• Symbol: Dicer1^tm1Gz
• Name: dicer 1, ribonuclease type III; targeted mutation 1, Genshi Zhao
• MGI ID: MGI:3576927
• Synonyms: Dicer^ex1/2
• Gene: Dicer1 Location: Chr12:104654001-104718211 bp, - strand Genetic Position: Chr12, 54.83 cM, cytoband E-F1
• Alliance: Dicer1^tm1Gz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97770
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1 and 2 were replaced with a neomycin resistance gene to create this severely hypomorphic allele. Although a transcript from these two exons was not present in Northern blot analyses, a transcript originating from exon 3 was found. Western blot demonstrated the presence of a nonfunctional protein product probably originating from exon 4. (J:97770)

Expression

In Mice Carrying this Mutation:	16 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dicer1 Mutation: 96 strains or lines available

References

• Original: J:97770 Yang WJ, et al., Dicer is required for embryonic angiogenesis during mouse development. J Biol Chem. 2005 Mar 11;280(10):9330-5
• All: 1 reference(s)