Sema5a^tm1Awp
Targeted Allele Detail

Summary

• Symbol: Sema5a^tm1Awp
• Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; targeted mutation 1, Andreas W Puschel
• MGI ID: MGI:3577050
• Synonyms: Sema5a^-
• Gene: Sema5a Location: Chr15:32244959-32696487 bp, + strand Genetic Position: Chr15, 13.02 cM
• Alliance: Sema5a^tm1Awp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97663
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exons 4 and 5 with a PGK-neo cassette. The null allele was achieved by inserting a stop codon after amino acid 121, thereby rendering a truncated protein unlikely to be biological active. Alternatively, aberrant splicing between exons 3 and 6 would result in the generation of a stop codon after amino acid 141. Western blot analysis of membrane fractions demonstrated a complete lack of protein in mutants. (J:97663)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sema5a Mutation: 67 strains or lines available

References

• Original: J:97663 Fiore R, et al., Inactivation of the Sema5a gene results in embryonic lethality and defective remodeling of the cranial vascular system. Mol Cell Biol. 2005 Mar;25(6):2310-9
• All: 1 reference(s)