Tcra-J^tm2.3Krg
Targeted Allele Detail

Summary

• Symbol: Tcra-J^tm2.3Krg
• Name: T cell receptor alpha, joining region; targeted mutation 2.3, Michael S Krangel
• MGI ID: MGI:3577336
• Synonyms: Delta5', Tcra^tm2.2Krg
• Gene: Tcra-J Location: unknown Genetic Position: Chr14, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97736
• Parent Cell Line: EF1 (ES Cell)
• Strain of Origin: 129S/SvEv x C57BL/6

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Mutation details: Tcra-J^tm2.1Krg ES cells (where the Trja49 promoter (Rr319) and gene segment were replaced with a loxP site flanked neomycin resistance gene cassette and the neo cassette was removed through subsequent cre-mediated recombination) were targeted with a vector replacing the TEA (T early alpha) promoter with a floxed neo cassette. The region between the loxP site in place of Trja49 and the upstream loxP site in place of the TEA promoter was deleted through subsequent cre-mediated recombination. This deletes segments Traj50-61 and the non-coding TEA exon. No gross deficiency was found in alphabeta TCR expression or alphabeta T cell development in mutant mice. (J:97736)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcra-J Mutation: 0 strains or lines available

References

• Original: J:97736 Hawwari A, et al., Regulation of T cell receptor alpha gene assembly by a complex hierarchy of germline Jalpha promoters. Nat Immunol. 2005 May;6(5):481-9
• All: 2 reference(s)