Grid2^{tm1(cre/PGR*)Ksak}
Targeted Allele Detail

Summary

• Symbol: Grid2^{tm1(cre/PGR*)Ksak}
• Name: glutamate receptor, ionotropic, delta 2; targeted mutation 1, Kenji Sakimura
• MGI ID: MGI:3577356
• Synonyms: D2CPR, GluRdelta2^CrePR, Grid2^{tm1(cre/PGR)Ksak}
• Gene: Grid2 Location: Chr6:63232860-64681307 bp, + strand Genetic Position: Chr6, 29.77 cM
• Alliance: Grid2^{tm1(cre/PGR*)Ksak} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:97754
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: mifepristone
• Mutations: Insertion, Intragenic deletion
• Grid2^{tm1(cre/PGR*)Ksak} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Grid2 (MGI:95813)

• Mutation details: The targeting vector consisting of a fusion protein of Cre recombinase plus a modified ligand binding domain of the human progesterone receptor and pgk-neo-polyA cassette was inserted in the translational initiation site of the Grid2 gene in frame. Approximately 900bp nucleotides from the initiation site of the endogenous sequence were deleted. The ligand-binding domain of human progesterone receptor was modified such that it binds and can be activated by the synthetic steroid, RU486, but not by the endogenous ligand, progesterone. Upon induction of inserted cre/PGR expression with RU486, activity was noted in mature Purkinje cells of the cerebellum. (J:97754)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Grid2 (mouse) Summary of all recombinase alleles driven by Grid2. Comparative matrix view of recombinase activities Alliance mouse Grid2 gene page

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grid2 Mutation: 85 strains or lines available

Notes

ES cell line = Bruce (no line given).

References

• Original: J:97754 Kitayama K, et al., Purkinje cell-specific and inducible gene recombination system generated from C57BL/6 mouse ES cells. Biochem Biophys Res Commun. 2001 Mar;281(5):1134-40
• All: 7 reference(s)