Tg(Igk-Lta/Ltb)4Cys
Transgene Detail

Summary

• Symbol: Tg(Igk-Lta/Ltb)4Cys
• Name: transgene insertion 4, Jason G Cyster
• MGI ID: MGI:3577756
• Synonyms: kappaLTalpha
• Transgene: Tg(Igk-Lta/Ltb)4Cys Location: unknown
• Alliance: Tg(Igk-Lta/Ltb)4Cys page

Transgene origin

• Strain of Origin: (C57BL/6J x DBA/2J)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Igk-Lta/Ltb)4Cys expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Lta (MGI:104797)
  mouse	Ltb (MGI:104796)

• Mutation details: A 450 bp DNA fragment of the Ltb gene coding the transmembrane and membrane-proximal region was fused to a 486 bp fragment of the Lta gene coding for the receptor binding domain. This chimeric construct was placed under the control of an Igk promoter. (J:73100)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Of the several lines created, lines 4 and 10 both overexpressed the fusion gene on B cells but not on T cells.

References

• Original: J:73100 Ngo VN, et al., Splenic T zone development is B cell dependent. J Exp Med. 2001 Dec 3;194(11):1649-60
• All: 3 reference(s)