Nr3c1^tm3Ljm
Targeted Allele Detail

Summary

• Symbol: Nr3c1^tm3Ljm
• Name: nuclear receptor subfamily 3, group C, member 1; targeted mutation 3, Louis J Muglia
• MGI ID: MGI:3577992
• Synonyms: GFP-GR
• Gene: Nr3c1 Location: Chr18:39543598-39652474 bp, - strand Genetic Position: Chr18, 21.09 cM
• Alliance: Nr3c1^tm3Ljm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98328
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed with a GFP fused to the ATG in exon 2 by a 5-amino acid linker, GGSGG. A PGK neo was included in intron 2 and was subsequently removed by crossing with Tg(EIIa-cre)C5379Lmgd mice. Western blot of heterozygotes demonstrated that expression of the GFP-GR fusion protein is equal to that of the endogenous protein. (J:98328)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nr3c1 Mutation: 35 strains or lines available

References

• Original: J:98328 Brewer JA, et al., Green fluorescent protein-glucocorticoid receptor knockin mice reveal dynamic receptor modulation during thymocyte development. J Immunol. 2002 Aug 1;169(3):1309-18
• All: 4 reference(s)