Notch3^tm1Khan
Targeted Allele Detail

Summary

• Symbol: Notch3^tm1Khan
• Name: notch 3; targeted mutation 1, Kazunori Hanaoka
• MGI ID: MGI:3578622
• Synonyms: Notch3^m
• Gene: Notch3 Location: Chr17:32339794-32385826 bp, - strand Genetic Position: Chr17, 17.37 cM
• Alliance: Notch3^tm1Khan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98284
• Parent Cell Line: TT2F (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 4 was disrupted by the insertion of a neomycin resistance gene. Exon 4 encodes part of the extracellular domain. Northern blot revealed a transcript shorter than wild-type. 5' RACE experiments revealed that the start site for this short transcript was 23bp downstream of the neo in exon 4. Western blot analysis using antibodies raised against the intracellular domain indicated that the allele is a null. (J:98284)

Expression

In Mice Carrying this Mutation:	94 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Notch3 Mutation: 94 strains or lines available

References

• Original: J:98284 Kitamoto T, et al., Functional redundancy of the Notch gene family during mouse embryogenesis: Analysis of Notch gene expression in Notch3-deficient mice. Biochem Biophys Res Commun. 2005 Jun 17;331(4):1154-62
• All: 3 reference(s)