Mfge8^{Gt(KST227)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Mfge8^{Gt(KST227)Byg}
• Name: milk fat globule EGF and factor V/VIII domain containing; gene trap KST227, BayGenomics
• MGI ID: MGI:3578644
• Synonyms: Mfge8^-, Mfge8^{Gt1(pGT1-pfs)Des}, Mfge8^{Gt(Betageo)1Zm}
• Gene: Mfge8 Location: Chr7:78783516-78798808 bp, - strand Genetic Position: Chr7, 44.9 cM
• Alliance: Mfge8^{Gt(KST227)Byg} page

Mutation origin

• Mutant Cell Line: KST227
• Germline Transmission: Earliest citation of germline transmission: J:98314
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1TMpfs Vector Type: secretory trap
• Mutation details: A gene trap vector, pGT1TMpfs, including a Betagalactosidase and a neomycin-resistance gene was inserted into the locus in intron 7, downstream of the C1 domain. The transcript resulting from the event encoded a fusion protein of the N-terminal sequence of the locus up to the C1 domain, followed by a TM domain and the beta-geo gene trap vector. (J:98314)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  KST227	CC144736 ; (MGI Seq Detail)	5' RACE	Chr7:78791393-78792300 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  5 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Mfge8 Mutation: 23 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:98314 Silvestre JS, et al., Lactadherin promotes VEGF-dependent neovascularization. Nat Med. 2005 Jun;11(6):499-506
• All: 26 reference(s)