Zfp36l2^tm1Pjb
Targeted Allele Detail

Summary

• Symbol: Zfp36l2^tm1Pjb
• Name: zinc finger protein 36, C3H type-like 2; targeted mutation 1, Perry J Blackshear
• MGI ID: MGI:3578661
• Gene: Zfp36l2 Location: Chr17:84491359-84495375 bp, - strand Genetic Position: Chr17, 55.02 cM, cytoband E4
• Alliance: Zfp36l2^tm1Pjb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98334
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector containing a PGK-neo was inserted into exon 1, 30 bp downstream from the ATG. Northern and Western blot analyses confirmed disruption of transcript and protein, respectively, from exon 1. A transcript was found that appeared to result from the fusion of exon 2 to 403 bp from the 3' end of the intron instead of to the neo transcript. A truncated protein was also found at significantly reduced levels than wild-type. (J:98334)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp36l2 Mutation: 22 strains or lines available

References

• Original: J:98334 Ramos SB, et al., The CCCH tandem zinc-finger protein Zfp36l2 is crucial for female fertility and early embryonic development. Development. 2004 Oct;131(19):4883-93
• All: 2 reference(s)