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Tg(Ins2-TFRC/OVA)296Wehi
Transgene Detail
Summary
Symbol: Tg(Ins2-TFRC/OVA)296Wehi
Name: transgene insertion 296, Walter and Eliza Hall Institute of Medical Research
MGI ID: MGI:3578729
Synonyms: RIP-mOVA, RIPmOVA
Transgene: Tg(Ins2-TFRC/OVA)296Wehi  Location: unknown  
Alliance: Tg(Ins2-TFRC/OVA)296Wehi page
Transgene
origin
Strain of Origin:  C57BL/6
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(Ins2-TFRC/OVA)296Wehi expresses 2 genes
 
Tg(Ins2-TFRC/OVA)296Wehi expression driven by 1 gene
 
Mutation detailsThe transgene encodes a fusion protein comprising the carboxy-terminal 118 amino acids of the human transferrin receptor, which include the cytoplasmic tail, membrane-targeting signal and anchor domain of the protein, joined to amino acids 139-385 of mature ovalbumin. Its expression is directed by the rat insulin 2 promoter. (J:98589)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Notes
Ovalbumin (OV) is present at high levels in pancreatic beta cells and in kidney proximal tubule cells and at very low levels in testes of transgenic mice. No OV has been detected immunohistologically in any of multiple other tissues examined. Mice expressing both this transgene and Tg(Tcra,Tcrb)1100Mjb (OT-1), which encodes an OV-specific T cell receptor expressed on CD8+ T lymphocytes, exhibit thymic deletion of OT-1 T cells, indicating presence of OV at very low levels in the thymus. Western blot analysis reproducibly measured the pancreatic islet OV content as 2.2 ng/ug of protein.
References
Original:  J:98589 Kurts C, et al., Constitutive class I-restricted exogenous presentation of self antigens in vivo. J Exp Med. 1996 Sep 1;184(3):923-30
All:  120 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory