Pram1^tm1Gak
Targeted Allele Detail

Summary

• Symbol: Pram1^tm1Gak
• Name: PML-RAR alpha-regulated adaptor molecule 1; targeted mutation 1, Gary A Koretzky
• MGI ID: MGI:3579812
• Synonyms: PRAM-1^-
• Gene: Pram1 Location: Chr17:33857030-33864680 bp, + strand Genetic Position: Chr17, 17.98 cM
• Alliance: Pram1^tm1Gak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95253
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced the start codon and 1,000 bp of the second coding exon. Immunoprecipitation and Western blot failed to detect protein in mutant bone marrow cell lysates. (J:95253)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pram1 Mutation: 30 strains or lines available

References

• Original: J:95253 Clemens RA, et al., PRAM-1 is required for optimal integrin-dependent neutrophil function. Mol Cell Biol. 2004 Dec;24(24):10923-32
• All: 1 reference(s)