Lmbr1^tm1Kng
Targeted Allele Detail

Summary

• Symbol: Lmbr1^tm1Kng
• Name: limb region 1; targeted mutation 1, David M Kingsley
• MGI ID: MGI:3579991
• Synonyms: Lmbr1^ATG
• Gene: Lmbr1 Location: Chr5:29434800-29583414 bp, - strand Genetic Position: Chr5, Syntenic
• Alliance: Lmbr1^tm1Kng page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72166
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 1.1 kb sequence containing 365 bp upstream of the predicted translational start site of exon 1 and 696 bp downstream of the exon 1 splice donor site was replaced with a PGKneo selection cassette. The deleted sequence was common to both known transcripts. Northern blot of mutant brains suggested that the allele may not have been a null as overexposure to probes demonstrated transcripts at ~7% of wild-type levels. (J:72166)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lmbr1 Mutation: 33 strains or lines available

References

• Original: J:72166 Clark RM, et al., Reciprocal mouse and human limb phenotypes caused by gain- and loss-of-function mutations affecting lmbr1. Genetics. 2001 Oct;159(2):715-26
• All: 1 reference(s)