Hnrnpu^{Gt(U3Neo)1Rul}
Gene trapped Allele Detail

Summary

• Symbol: Hnrnpu^{Gt(U3Neo)1Rul}
• Name: heterogeneous nuclear ribonucleoprotein U; gene trap 1, H Earl Ruley
• MGI ID: MGI:3580289
• Gene: Hnrnpu Location: Chr1:178148673-178165362 bp, - strand Genetic Position: Chr1, 83.16 cM
• Alliance: Hnrnpu^{Gt(U3Neo)1Rul} page

Mutation origin

• Mutant Cell Line: 1B3
• Germline Transmission: Earliest citation of germline transmission: J:98861
• Parent Cell Line: D3H (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector Vector: U3NeoSV1
• Mutation details: The gene trap vector inserted into an intron of the locus and resulted in alternative splicing to a cryptic 3' splice site located in the neo, creating a hypomorphic allele. The expected transcript would be 503 nt long, however, RT-PCR demonstrated that the major PCR product was only 93 nt long and did not hybridize to a U3 probe. (J:98861, J:151685)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  PSTVU01B3	CL440622 ; (MGI Seq Detail)	plasmid rescue	Chr1:178163209-178163774 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  1 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hnrnpu Mutation: 43 strains or lines available

References

• Original: J:98861 Roshon MJ, et al., Hypomorphic mutation in hnRNP U results in post-implantation lethality. Transgenic Res. 2005 Apr;14(2):179-92
• All: 2 reference(s)