Sphk2^{Gt(NPX039)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Sphk2^{Gt(NPX039)Byg}
• Name: sphingosine kinase 2; gene trap NPX039, BayGenomics
• MGI ID: MGI:3580701
• Synonyms: Sphk2^{Gt(pGT1dTMpfs)1Kly}
• Gene: Sphk2 Location: Chr7:45358891-45367426 bp, - strand Genetic Position: Chr7, 29.45 cM
• Alliance: Sphk2^{Gt(NPX039)Byg} page

Mutation origin

• Mutant Cell Line: NPX039
• Germline Transmission: Earliest citation of germline transmission: J:102911
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1dTMpfs Vector Type: secretory trap
• Mutation details: BayGenomics exon trap vector: pGT1dTMpfs (11611 nt). The trap element inserted 1.7 kbp downstream of the second exon. RT-PCR did not detect mRNA in kidney. (J:102911)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  NPX039	CC156084 ; (MGI Seq Detail)	5' RACE	Chr7:45366417-45367387 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  15 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sphk2 Mutation: 38 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:102911 Kharel Y, et al., Sphingosine kinase 2 is required for modulation of lymphocyte traffic by FTY720. J Biol Chem. 2005 Nov 4;280(44):36865-72
• All: 4 reference(s)