Kras^tm1(Hras)Rdl
Targeted Allele Detail

Summary

• Symbol: Kras^tm1(Hras)Rdl
• Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 1, Roberto Di Lauro
• MGI ID: MGI:3581451
• Synonyms: HrasKI, Kras^(Hras)
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^tm1(Hras)Rdl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98940
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A cassette containing Hras1 cDNA and a neo was fused in-frame with exon 2 such that synthesis of any Kras product would be abolished. Kras protein was undetectable in mutants. Hras1 protein was demonstrated to be present in brain, heart, liver, lung and spinal cord of mutants. (J:98940)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 84 strains or lines available

References

• Original: J:98940 Potenza N, et al., Replacement of K-Ras with H-Ras supports normal embryonic development despite inducing cardiovascular pathology in adult mice. EMBO Rep. 2005 May;6(5):432-7
• All: 5 reference(s)