About   Help   FAQ
Smad9tm1Mwst
Targeted Allele Detail
Summary
Symbol: Smad9tm1Mwst
Name: SMAD family member 9; targeted mutation 1, Michael Weinstein
MGI ID: MGI:3581766
Synonyms: Smad83loxP
Gene: Smad9  Location: Chr3:54663003-54708678 bp, + strand  Genetic Position: Chr3, 25.62 cM, cytoband D
Alliance: Smad9tm1Mwst page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:99098
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Conditional ready, Hypomorph)
Mutation:    Insertion
 
Mutation detailsA targeting construct was designed to flank exon 3 with loxP sites and insert a PGK-neo into intron 3 with a 3' loxP site. RT-PCR demonstrated a hybrid transcript in mutant mice, utilizing a splice site 5 bp upstream of the initiationg ATG. This aberrant splicing caused neo to be out of frame with the endogenous transcript, resulting in a premature stop codon, and thereby allowing synthesis of a truncated Smad9 protein lacking about three-fourths of the MH2 domain. Real time ePCR demonstrated that transcript is reduced in the adult mutant brain, and that this allele is a hypomorph. (J:99098)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 8 assay results
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Smad9 Mutation:  32 strains or lines available
References
Original:  J:99098 Hester M, et al., Smad1 and Smad8 function similarly in mammalian central nervous system development. Mol Cell Biol. 2005 Jun;25(11):4683-92
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
11/12/2024
MGI 6.24
The Jackson Laboratory