Smad9tm1Mwst
Targeted Allele Detail
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Symbol: |
Smad9tm1Mwst |
Name: |
SMAD family member 9; targeted mutation 1, Michael Weinstein |
MGI ID: |
MGI:3581766 |
Synonyms: |
Smad83loxP |
Gene: |
Smad9 Location: Chr3:54663003-54708678 bp, + strand Genetic Position: Chr3, 25.62 cM, cytoband D
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Alliance: |
Smad9tm1Mwst page
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Germline Transmission: |
Earliest citation of germline transmission:
J:99098
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Parent Cell Line: |
TC1/TC-1 (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Conditional ready, Hypomorph) |
Mutation: |
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Insertion
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Mutation details: A targeting construct was designed to flank exon 3 with loxP sites and insert a PGK-neo into intron 3 with a 3' loxP site. RT-PCR demonstrated a hybrid transcript in mutant mice, utilizing a splice site 5 bp upstream of the initiationg ATG. This aberrant splicing caused neo to be out of frame with the endogenous transcript, resulting in a premature stop codon, and thereby allowing synthesis of a truncated Smad9 protein lacking about three-fourths of the MH2 domain. Real time ePCR demonstrated that transcript is reduced in the adult mutant brain, and that this allele is a hypomorph.
(J:99098)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Smad9 Mutation: |
32 strains or lines available
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Original: |
J:99098 Hester M, et al., Smad1 and Smad8 function similarly in mammalian central nervous system development. Mol Cell Biol. 2005 Jun;25(11):4683-92 |
All: |
1 reference(s) |
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