Arhgef1^tm1Kdf
Targeted Allele Detail

Summary

• Symbol: Arhgef1^tm1Kdf
• Name: Rho guanine nucleotide exchange factor 1; targeted mutation 1, Klaus-Dieter Fischer
• MGI ID: MGI:3582489
• Synonyms: Lsc^-
• Gene: Arhgef1 Location: Chr7:24602337-24626019 bp, + strand Genetic Position: Chr7, 13.52 cM
• Alliance: Arhgef1^tm1Kdf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99463
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin cassette replaced bp 1513-1651 including exon sequences of the DH domain encoding for amino acid residues 476-521. Immunoblot analysis of total spleen cell lysates revealed that homozygous mutants do not express protein. (J:99463)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arhgef1 Mutation: 73 strains or lines available

References

• Original: J:99463 Girkontaite I, et al., Lsc is required for marginal zone B cells, regulation of lymphocyte motility and immune responses. Nat Immunol. 2001 Sep;2(9):855-62
• All: 2 reference(s)