Rpa1^tm1Wed
Targeted Allele Detail

Summary

• Symbol: Rpa1^tm1Wed
• Name: replication protein A1; targeted mutation 1, Winfried Edelmann
• MGI ID: MGI:3582609
• Synonyms: Rpa1^689C, Rpa1^L230P
• Gene: Rpa1 Location: Chr11:75191085-75239478 bp, - strand Genetic Position: Chr11, 45.79 cM
• Alliance: Rpa1^tm1Wed page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99303
• Parent Cell Line: WW6 (ES Cell)
• Strain of Origin: STOCK 129/Sv and C57BL/6J and SJL

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Single point mutation
• Mutation details: A targeting vector was designed to insert a t689c transition, causing an L230P substitution. RT-PCR and sequence analysis confirmed successful targeting. Mutant protein was present in heterozygous mutant MEFs at wild-type levels. (J:99303)

Tumor Data

List all tumor models in MMHCdb carrying Rpa1^tm1Wed

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rpa1 Mutation: 54 strains or lines available

References

• Original: J:99303 Wang Y, et al., Mutation in Rpa1 results in defective DNA double-strand break repair, chromosomal instability and cancer in mice. Nat Genet. 2005 Jul;37(7):750-5
• All: 2 reference(s)