Gast^tm1(INS)Ez
Targeted Allele Detail

Summary

• Symbol: Gast^tm1(INS)Ez
• Name: gastrin; targeted mutation 1, Elena Zhukova
• MGI ID: MGI:3582613
• Synonyms: G-InsKi
• Gene: Gast Location: Chr11:100225233-100227822 bp, + strand Genetic Position: Chr11, 63.46 cM
• Alliance: Gast^tm1(INS)Ez page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99270
• Parent Cell Line: LW1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Insertion
• Mutation details: The human insulin cDNA, without the polyA sequence, and a floxed pgk-neo cassette were inserted into exon 2. The human insulin gene is thus placed under the control of the mouse gastrin promoter. (J:99270)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gast Mutation: 18 strains or lines available

References

• Original: J:99270 Lu YC, et al., Release of transgenic human insulin from gastric g cells: a novel approach for the amelioration of diabetes. Endocrinology. 2005 Jun;146(6):2610-9
• All: 2 reference(s)