Gata1^tm8.1Sho
Targeted Allele Detail

Summary

• Symbol: Gata1^tm8.1Sho
• Name: GATA binding protein 1; targeted mutation 8.1, Stuart H Orkin
• MGI ID: MGI:3582685
• Synonyms: Gata1^deltaefl
• Gene: Gata1 Location: ChrX:7825504-7842844 bp, - strand Genetic Position: ChrX, 3.59 cM, cytoband A2
• Alliance: Gata1^tm8.1Sho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99310
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An AvrII-NheI genomic fragment spanning exon 2 and part of exon 3 was amplified. Then a 5' loxP site was introduced to the AvrII site and a neo cassette was inserted into the PstI site in the intron between exons 2 and 3. The neo cassette was later removed by transfecting a GFP-Cre plasmid and then carrying out fluorescence-activated cell sorting for GFP+ cells, leaving exon 2 flanked by loxP sites. (J:99310)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Gata1^tm8.1Sho/Gata1^tm8.1Sho	involves: 129S1/Sv * C57BL/6
ot2	Gata1^tm8.1Sho/Y	involves: 129S1/Sv * C57BL/6

Phenotypes:

Affected Systems	hm1	ot2
show or hide all annotated terms Sex:
hematopoietic system	√	√
abnormal megakaryocyte morphology	√	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gata1 Mutation: 45 strains or lines available

References

• Original: J:99310 Li Z, et al., Developmental stage-selective effect of somatically mutated leukemogenic transcription factor GATA1. Nat Genet. 2005 Jun;37(6):613-9
• All: 1 reference(s)