Gata1^tm8.1Sho
Targeted Allele Detail

Summary

• Symbol: Gata1^tm8.1Sho
• Name: GATA binding protein 1; targeted mutation 8.1, Stuart H Orkin
• MGI ID: MGI:3582685
• Synonyms: Gata1^deltaefl
• Gene: Gata1 Location: ChrX:7825504-7842844 bp, - strand Genetic Position: ChrX, 3.59 cM, cytoband A2
• Alliance: Gata1^tm8.1Sho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99310
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An AvrII-NheI genomic fragment spanning exon 2 and part of exon 3 was amplified. Then a 5' loxP site was introduced to the AvrII site and a neo cassette was inserted into the PstI site in the intron between exons 2 and 3. The neo cassette was later removed by transfecting a GFP-Cre plasmid and then carrying out fluorescence-activated cell sorting for GFP+ cells, leaving exon 2 flanked by loxP sites. (J:99310)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gata1 Mutation: 45 strains or lines available

References

• Original: J:99310 Li Z, et al., Developmental stage-selective effect of somatically mutated leukemogenic transcription factor GATA1. Nat Genet. 2005 Jun;37(6):613-9
• All: 1 reference(s)