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Hprt1tm2(Pgk1-Pac/TK)Brd
Targeted Allele Detail
Summary
Symbol: Hprt1tm2(Pgk1-Pac/TK)Brd
Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 2, Allan Bradley
MGI ID: MGI:3582838
Synonyms: puDeltatk
Gene: Hprt1  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance: Hprt1tm2(Pgk1-Pac/TK)Brd page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:99258
Parent Cell Line:  AB1 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprt1+
Mutation
description
Allele Type:    Targeted (Conditional ready, Inserted expressed sequence)
Mutation:    Insertion
 
Mutation detailsThe coding sequence of the transgene consists of the puromycin acetyltransferase coding sequence fused in-frame to a 5' truncated HSV1 thymidine kinase gene that begins at the second ATG of the full-length gene and encodes a protein lacking the carboxy-terminal 45 amino acids. This construct resides upstream of the bovine growth hormone polyadenylation signal and downstream of the phosphoglycerate kinase 1 promoter followed by a loxP-flanked neomycin acetyltransferase gene with the bovine growth hormone poly-A signal. The transgene was inserted in reverse orientation into exon 3 of the endogenous Hprt gene via homologous recombination. (J:99258, J:99558)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:  1279 strains or lines available
Notes
When mice bearing this transgene are crossed with mice transgenic for cre, excision of the loxP-flanked neo gene in cells expressing Cre recombinase results in expression of a puromycin acetyltransferase-thymidine kinase fusion protein under control of the (ubiquitously-expressing) Pgk1 promoter. Treatment of the mice with ganciclovir (GCV) causes specific ablation of cells expressing HSV1 Tk activity.
References
Original:  J:99258 Chen YT, et al., Bigenic Cre/loxP, puDeltatk conditional genetic ablation. Nucleic Acids Res. 2004;32(20):e161
All:  5 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory