Rec8^tm1Mjm
Targeted Allele Detail

Summary

• Symbol: Rec8^tm1Mjm
• Name: REC8 meiotic recombination protein; targeted mutation 1, Michael J McKay
• MGI ID: MGI:3583574
• Synonyms: Rec8^-
• Gene: Rec8 Location: Chr14:55855494-55862852 bp, + strand Genetic Position: Chr14, 28.19 cM
• Alliance: Rec8^tm1Mjm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99402
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct was designed to replace 19 coding exons with a loxP-flanked cassette containing GFP and a neomycin resistance cassette. Testes from mutants were demonstrated to lack mRNA by Northern blot analysis. (J:99402)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rec8 Mutation: 25 strains or lines available

References

• Original: J:99402 Xu H, et al., Absence of mouse REC8 cohesin promotes synapsis of sister chromatids in meiosis. Dev Cell. 2005 Jun;8(6):949-61
• All: 4 reference(s)