Gna13^tm2Cgh
Targeted Allele Detail

Summary

• Symbol: Gna13^tm2Cgh
• Name: guanine nucleotide binding protein, alpha 13; targeted mutation 2, Shaun R Coughlin
• MGI ID: MGI:3583876
• Synonyms: Galpha13^flox
• Gene: Gna13 Location: Chr11:109253657-109292195 bp, + strand Genetic Position: Chr11, 71.88 cM
• Alliance: Gna13^tm2Cgh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99731
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 4 and much of intron 3 were replaced via homologous recombination with a construct composed of an frt flanked neomycin resistance cassette, a floxed exon 4, and a segment of intron 4 equivalent to the region of the endogenous intron being replaced. Mice carrying this targeted allele were crossed to transgenic mice carrying Flp recombinase under the control of a beta-actin promoter. This results in excision of the neomycin cassette and leaves a floxed exon 4. (J:99731)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gna13 Mutation: 12 strains or lines available

References

• Original: J:99731 Ruppel KM, et al., Essential role for Galpha13 in endothelial cells during embryonic development. Proc Natl Acad Sci U S A. 2005 Jun 7;102(23):8281-6
• All: 9 reference(s)