Polh^tm1Fmh
Targeted Allele Detail

Summary

• Symbol: Polh^tm1Fmh
• Name: polymerase (DNA directed), eta (RAD 30 related); targeted mutation 1, Fumio Hanaoka
• MGI ID: MGI:3583930
• Gene: Polh Location: Chr17:46482281-46513567 bp, - strand Genetic Position: Chr17, 22.9 cM, cytoband C
• Alliance: Polh^tm1Fmh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99721
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into exon 8 and gives rise to a stop codon within the neomycin gene, truncating any potential protein product before the pol catalytic domains. Western blot analysis failed to detect truncated protein. (J:99721)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polh Mutation: 38 strains or lines available

References

• Original: J:99721 Martomo SA, et al., Different mutation signatures in DNA polymerase eta- and MSH6-deficient mice suggest separate roles in antibody diversification. Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8656-61
• All: 3 reference(s)