Rrn3^tm1.2Igt
Targeted Allele Detail

Summary

• Symbol: Rrn3^tm1.2Igt
• Name: RRN3 RNA polymerase I transcription factor homolog (yeast); targeted mutation 1.2, Ingrid Grummt
• MGI ID: MGI:3584037
• Synonyms: TIF-1A^-
• Gene: Rrn3 Location: Chr16:13598572-13632703 bp, + strand Genetic Position: Chr16, 9.59 cM
• Alliance: Rrn3^tm1.2Igt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99711
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: An FRP-flanked neomycin resistance cassette downstream of a loxP site was inserted into intron 11 and a second loxP site was inserted in to intron 14 via homologous recombination. Heterozygous mice were bred with Tg(CMV-cre)1Cgn carriers to excise exons 12-14. RT-PCR analysis indicated that no detectable transcript was expressed from this allele. (J:99711)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rrn3 Mutation: 42 strains or lines available

References

• Original: J:99711 Yuan X, et al., Genetic inactivation of the transcription factor TIF-IA leads to nucleolar disruption, cell cycle arrest, and p53-mediated apoptosis. Mol Cell. 2005 Jul 1;19(1):77-87
• All: 2 reference(s)