Dgki^tm1Mtop
Targeted Allele Detail

Summary

• Symbol: Dgki^tm1Mtop
• Name: diacylglycerol kinase, iota; targeted mutation 1, Matthew K Topham
• MGI ID: MGI:3584141
• Synonyms: Dgki^-
• Gene: Dgki Location: Chr6:36822957-37277119 bp, - strand Genetic Position: Chr6, 15.6 cM
• Alliance: Dgki^tm1Mtop page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99817
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of the catalytic domain encoded by exon 14 was replaced by a neomycin resistance gene in reverse orientation. RT-PCR confirmed that no full-length or truncated transcript was expressed in mutant liver tissue. (J:99817)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dgki Mutation: 39 strains or lines available

References

• Original: J:99817 Regier DS, et al., Diacylglycerol kinase iota regulates Ras guanyl-releasing protein 3 and inhibits Rap1 signaling. Proc Natl Acad Sci U S A. 2005 May 24;102(21):7595-600
• All: 4 reference(s)