Gt(ROSA)26Sor^{tm1(rtTA2S*M2)Whsu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(rtTA2S*M2)Whsu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Wei Hsu
• MGI ID: MGI:3584142
• Synonyms: Gt(ROSA)26Sor^{tm1(xstpx-rtTA2S*M2)Whsu}, R26STOPrtTA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(rtTA2S*M2)Whsu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99718
• Parent Cell Line: CSL3 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Transactivator)
• Mutation: Insertion
• Mutation details: A synthetic modified version of the rtTA gene preceded by a loxP flanked STOP sequence was inserted into the Gt(ROSA)26Sor locus. This version of rtTA contains 5 amino acid changes in the TetR moiety (S12G, E19G, A56P, D148E, and H179R) and a synthetic optimized transactivating domain. This rtTA has reduced basal activity and enhanced doxycycline sensitivity compared to wild-type rtTA. (J:99718)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:99718 Yu HM, et al., Development of a unique system for spatiotemporal and lineage-specific gene expression in mice. Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8615-20
• All: 4 reference(s)