Gt(ROSA)26Sor^{tm1.1(rtTA2S*M2)Whsu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(rtTA2S*M2)Whsu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Wei Hsu
• MGI ID: MGI:3584145
• Synonyms: R26rtTA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(rtTA2S*M2)Whsu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99718
• Parent Cell Line: CSL3 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Transactivator)
• Mutation: Insertion
• Mutation details: A synthetic, modified version of the rtTA gene preceded by a loxP flanked STOP sequence was inserted into the Gt(ROSA)26Sor locus. Carriers were bred with mice transgenic for cre recombinase under the control of beta actin, excising the STOP sequence and leaving rtTA under the endogenous Gt(ROSA)26Sor promoter. This version of rtTA contains 5 amino acid changes in the TetR moiety (S12G, E19G, A56P, D148E, and H179R) and a synthetic optimized transactivating domain. This rtTA has reduced basal activity and enhanced doxycycline sensitivity compared to wild-type rtTA. (J:99718)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 967 strains or lines available

References

• Original: J:99718 Yu HM, et al., Development of a unique system for spatiotemporal and lineage-specific gene expression in mice. Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8615-20
• All: 11 reference(s)