Tg(Amelx)42Msnd
Transgene Detail

Summary

• Symbol: Tg(Amelx)42Msnd
• Name: transgene insertion 42, Malcolm L Snead
• MGI ID: MGI:3584497
• Synonyms: M180deltaA-FLAG
• Transgene: Tg(Amelx)42Msnd Location: unknown
• Alliance: Tg(Amelx)42Msnd page

Transgene origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1 x CBA/J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Amelx)42Msnd expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Amelx (MGI:88005)

• Mutation details: The transgene consists of 2.3 kb of genomic DNA containing the amelogenin promoter region and the untranslated first exon, approximately 1.3 kb of intron 1, and the coding sequence for amelogenin - including the signal peptide necessary for secretion of the protein into the extracellular enamel space - with a 27-amino acid sequence containing the FLAG epitope replacing amino acids 3-42. The amelogenin stop codon is followed by the luciferase gene with its polyadenylation signal. (J:100004)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

In situ hybridization with a luciferase cDNA probe detects transgene mRNA only in ameloblasts. Immunohistochemical analysis of four day old incisors using antibodies to the FLAG epitope finds tagged amelogenin restricted to ameloblasts, where it is localized to the cytoplasm, and in the secreted organic enamel matrix. (J:100004)

Several independent founder lines generated from this construct were established; no differences were noted among their phenotypes, and no distinction was made among them.

References

• Original: J:100004 Paine ML, et al., Enamel biomineralization defects result from alterations to amelogenin self-assembly. J Struct Biol. 2000 Dec;132(3):191-200
• All: 6 reference(s)