Ilf3^tm1Pnk
Targeted Allele Detail

Summary

• Symbol: Ilf3^tm1Pnk
• Name: interleukin enhancer binding factor 3; targeted mutation 1, Peter N Kao
• MGI ID: MGI:3586304
• Synonyms: NF90^-
• Gene: Ilf3 Location: Chr9:21279167-21316657 bp, + strand Genetic Position: Chr9, 7.78 cM, cytoband A4-A5
• Alliance: Ilf3^tm1Pnk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99935
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene replaced exons 2-4, resulting in the deletion of the ATG codon and the introduction of a frameshift that disrupted the ORF. Northern blot of mutants confirmed recombination, and Western blot demonstrated complete lack of protein in mutants. (J:99935)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ilf3 Mutation: 77 strains or lines available

References

• Original: J:99935 Shi L, et al., NF90 regulates cell cycle exit and terminal myogenic differentiation by direct binding to the 3'-untranslated region of MyoD and p21WAF1/CIP1 mRNAs. J Biol Chem. 2005 May 13;280(19):18981-9
• All: 3 reference(s)