Nmt1^Gt(XE400)Byg
Gene trapped Allele Detail

Summary

• Symbol: Nmt1^Gt(XE400)Byg
• Name: N-myristoyltransferase 1; gene trap XE400, BayGenomics
• MGI ID: MGI:3586313
• Synonyms: Nmt1^-, Nmt1^{Gt(pGT1lxf)1Sgy}
• Gene: Nmt1 Location: Chr11:102919163-102959734 bp, + strand Genetic Position: Chr11, 66.71 cM, cytoband D
• Alliance: Nmt1^Gt(XE400)Byg page

Mutation origin

• Mutant Cell Line: XE400
• Germline Transmission: Earliest citation of germline transmission: J:99794
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A gene trap vector, pGT1Lxt, designed to create an in-frame fusion between the 5' exons of the locus and betagalactosidase and neomycin resistance genes was inserted into intron 3. Northern blot confirmed the insertion of the vector and the presence of a fusion transcript. (J:99932)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE400	CC183501 ; (MGI Seq Detail)	5' RACE	Chr11:102919373-102937346 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

13 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nmt1 Mutation: 55 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:99932 Yang SH, et al., N-myristoyltransferase 1 is essential in early mouse development. J Biol Chem. 2005 May 13;280(19):18990-5
• All: 4 reference(s)