Eae32^C57BL/10SnSg
QTL Variant Detail

Summary

• QTL variant: Eae32^C57BL/10SnSg
• Name: experimental allergic encephalomyelitis susceptibility 32; C57BL/10SnSg
• MGI ID: MGI:3586465
• QTL: Eae32 Location: Chr15:32578416-103425899 bp Genetic Position: Chr15, Syntenic

Variant origin

• Strain of Specimen: C57BL/10SnSg

Variant description

• Allele Type: QTL
• Mutation: Undefined
• Mutation details: This allele confers increased change in cortical motor evoked potential (cMEP) latency 0-15 days after EAE inoculation compared to SJL/J. (J:99793)
• Inheritance: Dominant

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:99793

Linkage analysis was performed on 400 animals from a (B10.S x SJL/J)F2 intercross to map QTL associated with susceptibility to experimental autoimmune encephalomyelitis (EAE). Parental strain SJL/J is susceptible to EAE whereas parental strain B10.S is resistant. Animals were immunized at 8-12 weeks of age to induce EAE. 150 informative microsatellite markers at an average spacing of 10 cM were used for the genome scan. Several new EAE loci were identified in this experiment.

Eae30 mapped to 22 cM on mouse Chromosome 1 near D1Mit303. The QTL interval of Eae30 spans 11 cM - 32 cM and is flanked by D1Mit380 and D1Mit236. This locus shows suggestive linkage to forelimb cortical motor evoked potential (cMEP) latency 15 days after inoculation (LOD=3.4). Linkage reaches statistical significance when EAE severity is used as a covariance (LOD=3.7). SJL/J-derived alleles at Eae30 confer increased forelimb cMEP latency. Eae30 overlaps with a previously identified diabetes QTL named Idd5 (40 cM) and partially overlaps with a previously identified QTL for demyelinating disease named Tmevd9 (32.8 cM). Potential candidate genes for Eae30 include Ctla4 (30.1 cM) and Icos (32 cM).

Eae31 mapped to 42 cM on mouse Chromosome 8 near D8Mit258. The QTL interval of Eae31 spans 32 cM - 48 cM and is flanked by D8Mit24 and D8Mit88. This locus shows linkage to forelimb cortical motor evoked potential (cMEP) latency before inoculation (LOD=6.99). SJL/J-derived alleles at Eae31 confer increased forelimb cMEP latency with a dominant mode of inheritance. Potential candidate genes for Eae31 are Cacna1a (38.5 cM), Casp3 (26 cM), Jak3 (33 cM), and Cpe (32.6 cM). Eae36 maps to 26 cM on mouse Chromosome 8 near D8Mit259. The QTL interval of Eae36 spans 2 cM - 34 cM and is flanked by D8Mit124 and D8Mit178. This locus is linked to T-lymphocyte infiltration in the spinal cord (LOD=4.1) and overlaps with a previously identified EAE QTL named Eae14 (21 cM). SJL/J-derived alleles at Eae36 confer increased T-lymphocyte infiltration of the spinalcord.

Eae32 mapped to 38 cM on mouse Chromosome 15 near D15Mit67. The QTL interval of Eae32 spans 24 cM - 56 cM and is flanked by D15Mit126 and D15Mit35. This locus shows linkage to the change in hindlimb cortical motor evoked potential (cMEP) latency 0- 15 days after inoculation. B10.S-derived alleles confer increased hindlimb cMEP latency with a dominant mode of inheritance. Potential candidate genes for Eae32 are Cacng2 (45.2 cM) and Prph1 (55.5 cM).

Eae33 mapped to 20 cM on mouse Chromosome 2 nearD2Mit32. The QTL interval of Eae33 spans 6 cM - 30 cM and is flanked by D2Mit372 and D2Mit11. This locus shows linkage to EAE severity in female animals (LOD=3.7) with the B10.S-derived allele conferring increased EAE severity. A potential candidate genefor Eae33 is Hc (23.5 cM).

Eae34 mapped to 56 cM - 59 cM on mouse Chromosome 10 near D10Mit223 and D10Mit271. The QTL interval of Eae34 spans approximately 42 cM - 58 cM and is flanked by D10Mit2 and D10Mit233. This locus shows linkage to EAE onset (LOD=3.7) and severity (LOD=3.8) in female animals. B10.S-derived alleles at Eae34 confer earlier onset of EAE and increased disease severity in female animals. Eae34 partially overlaps with a previously identified EAE QTL named Eae17 (36 cM).

Eae35 mapped to 68 cM on mouse Chromosome 18 near D18Mit144. The QTL interval of Eae35 spans 54 cM - 69 cM. This locus shows suggestive linkage to EAE severity in female animals (LOD=3.2) with B10.S-derived alleles conferring increased EAE severity. Eae35 overlaps witha previously identified EAE QTL named Eae25 (54 cM).

Eae37 mapped to 69 cM on mouse Chromosome 15 near D15Mit35. The QTL interval of Eae37 spans 58 cM - 69 cM. This locus is linked to microglia infiltration in the spinal cord (LOD=3.6). B10.S-derived alleles at Eae37 confer increased microglia infiltration in the spinal cord. Eae38 mapped to 54 cM - 56 cM on mouse Chromosome 15 near D15Mit171. The QTL interval of Eae38 spans approximately 18 cM - 72 cM. This locus shows suggestive linkage to AUC (area under curve) for B-lymphocyte infiltration in the spinal cord in male animals (LOD=3.3). B10.S-derived alleles at Eae38 confer increased AUC for B-lymphocyte infiltration in male animals.

References

• Original: J:99793 Mazon Pelaez I, et al., Identification of quantitative trait loci controlling cortical motor evoked potentials in experimental autoimmune encephalomyelitis: correlation with incidence, onset and severity of disease. Hum Mol Genet. 2005 Jul 15;14(14):1977-89
• All: 1 reference(s)