Tg(TcraCl4,TcrbCl4)1Shrm
Transgene Detail

Summary

• Symbol: Tg(TcraCl4,TcrbCl4)1Shrm
• Name: transgene insertion 1, Linda Sherman
• MGI ID: MGI:3586582
• Synonyms: C4, CL-4, CL4, CL-4 TCR, CL4-TCR, Clone 4, clone 4 TCR, Clone-4 TCR, (HA)-TCR
• Transgene: Tg(TcraCl4,TcrbCl4)1Shrm Location: unknown
• Alliance: Tg(TcraCl4,TcrbCl4)1Shrm page

Transgene origin

• Strain of Origin: (C57BL/6 x BALB/c)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TcraCl4,TcrbCl4)1Shrm expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: Tcra and Tcrb cDNAs were isolated from Clone-4, a CTL line derived from a B10.D2-Hc¹ H2^d H2-t18^c/nSnJ mouse immunized with influenza virus A/PR/8. A Tcra cDNA fragment representing the V-J exon, containing Valpha10, was cloned into a Tcra shuttle vector that includes a mouse Tcra-V promoter, the immunoglobulin heavy-chain enhancer E_H, and the Tcra-C genes with some flanking DNA. Likewise, a Tcrb cDNA fragment representing the VDJ exon, containing Vbeta8.2, was cloned into a Tcrb shuttle vector including a mouse Tcrb-V promoter, E_H, and the Tcrb-C genes with some flanking DNA. The plasmid constructs were coinjected. This transgenic TCR specifically recognizes influenza virus A/PR/8 hemagglutinin restricted by MHC class I H2k^d. (J:90982, J:99756)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

References

• Original: J:99756 Morgan DJ, et al., CD8(+) T cell-mediated spontaneous diabetes in neonatal mice. J Immunol. 1996 Aug 1;157(3):978-83
• All: 86 reference(s)