Ube2d2a^tm1Ssw
Targeted Allele Detail

Summary

• Symbol: Ube2d2a^tm1Ssw
• Name: ubiquitin-conjugating enzyme E2D 2A; targeted mutation 1, Simon S Wing
• MGI ID: MGI:3586923
• Synonyms: UBC4^-
• Gene: Ube2d2a Location: Chr18:35904612-35940225 bp, + strand Genetic Position: Chr18, 19.24 cM
• Alliance: Ube2d2a^tm1Ssw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100020
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence encoding amino acid residues 2 to 126 were replaced with a neomycin resistance cassette, removing 124 of 147 residues, including all of the elements making up the active site. Northern blot confirmed absence of wild-type transcript in mutant testis, but showed the presence of a longer transcript resulting from transcription through the larger neomycin resistance cassette. Western blot demonstrated absence of protein in mutant testis. (J:100020)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ube2d2a Mutation: 14 strains or lines available

Notes

ES cell line = RS1.

References

• Original: J:100020 Bedard N, et al., Mice lacking the UBC4-testis gene have a delay in postnatal testis development but normal spermatogenesis and fertility. Mol Cell Biol. 2005 Aug;25(15):6346-54
• All: 1 reference(s)