Phc2^tm1Hko
Targeted Allele Detail

Summary

• Symbol: Phc2^tm1Hko
• Name: polyhomeotic 2; targeted mutation 1, Haruhiko Koseki
• MGI ID: MGI:3586989
• Synonyms: Phc2^-
• Gene: Phc2 Location: Chr4:128548495-128646674 bp, + strand Genetic Position: Chr4, 62.12 cM
• Alliance: Phc2^tm1Hko page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100027
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exons 2 and 3 with a neomycin resistance cassette, resulting in the loss of the HDI region from the 36-kDa isoform. Northern blot showed the presence of three aberrant transcripts in mutant. However, immunoblot did not detect protein. (J:100027)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Phc2 Mutation: 83 strains or lines available

References

• Original: J:100027 Isono K, et al., Mammalian polyhomeotic homologues phc2 and phc1 act in synergy to mediate polycomb repression of hox genes. Mol Cell Biol. 2005 Aug;25(15):6694-706
• All: 6 reference(s)