Cux1^tm1Rhsc
Targeted Allele Detail

Summary

• Symbol: Cux1^tm1Rhsc
• Name: cut-like homeobox 1; targeted mutation 1, Richard H Scheuermann
• MGI ID: MGI:3586992
• Synonyms: cux/CDP^deltaHD
• Gene: Cux1 Location: Chr5:136276989-136596344 bp, - strand Genetic Position: Chr5, 75.96 cM
• Alliance: Cux1^tm1Rhsc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100055
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace the sequence encoding the homeodomain in exons 20 and 21 with a neomycin resistance gene. Immunoblot of mutant thymic nuclear extracts confirmed absence of wild-type protein and presence of a smaller protein equivalent to the predicted size of the mutant protein containing only 4 amino acids of the homeodomain and a deletion of the C-terminal repression domain. This band was present at a reduced intensity compared to the wild-type band in controls, suggesting the remaining protein has partial function and is a hypomorph. (J:100055)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cux1 Mutation: 112 strains or lines available

References

• Original: J:100055 Sinclair AM, et al., Lymphoid apoptosis and myeloid hyperplasia in CCAAT displacement protein mutant mice. Blood. 2001 Dec 15;98(13):3658-67
• All: 4 reference(s)