Klb^tm1Yin
Targeted Allele Detail

Summary

• Symbol: Klb^tm1Yin
• Name: klotho beta; targeted mutation 1, Yo-ichi Nabeshima
• MGI ID: MGI:3587748
• Synonyms: beta-kl^-, Betaklotho^-
• Gene: Klb Location: Chr5:65505657-65541350 bp, + strand Genetic Position: Chr5, 33.64 cM
• Alliance: Klb^tm1Yin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100209
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of the putative exon 1 containing the first ATG was deleted upon the insertion of an EGFP with a loxP-flanked PGK-neo in reverse orientation. Northern blot and RT-PCR confirmed lack of transcript in mutant liver samples. Absence of protein was demonstrated by Western blot of mutant liver, white adipose tissue and kidney. (J:100209)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Klb Mutation: 58 strains or lines available

References

• Original: J:100209 Ito S, et al., Impaired negative feedback suppression of bile acid synthesis in mice lacking betaKlotho. J Clin Invest. 2005 Aug 1;115(8):2202-2208
• All: 9 reference(s)