Atg7^tm1Tchi
Targeted Allele Detail

Summary

• Symbol: Atg7^tm1Tchi
• Name: autophagy related 7; targeted mutation 1, Tomoki Chiba
• MGI ID: MGI:3587769
• Synonyms: Atg7^F, Atg7^Flox, ATG7^flox
• Gene: Atg7 Location: Chr6:114620075-114837565 bp, + strand Genetic Position: Chr6, 53.05 cM, cytoband E3
• Alliance: Atg7^tm1Tchi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100199
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting construct was designed to insert a loxP site in intron of the 13th coding exon. Coding exon 14 was fused to a cDNA encoding coding exons 15, 16, and 17 together with a polyadenylation signal after the stop codon. This was then followed by a neomycin resistance cassette and a second loxP site. An additional exon 14 sequence with a stop codon preceding the active site cysteine codon was inserted after the second loxP site. Immunoblot showed that the presence of the loxP sites and neo did not interefere with translation. (J:100199)

Disease models

Expression

In Mice Carrying this Mutation:	9 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	17 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Atg7^tm1Tchi

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Atg7 Mutation: 51 strains or lines available

References

• Original: J:100199 Komatsu M, et al., Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol. 2005 May 9;169(3):425-34
• All: 349 reference(s)