Mrtfb^{Gt(RRJ478)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Mrtfb^{Gt(RRJ478)Byg}
• Name: myocardin related transcription factor B; gene trap RRJ478, BayGenomics
• MGI ID: MGI:3587937
• Synonyms: Mkl2^{Gt(pGT0Lxf)1Ucd}, MRTF-B^delta
• Gene: Mrtfb Location: Chr16:13074345-13235393 bp, + strand Genetic Position: Chr16, 9.24 cM
• Alliance: Mrtfb^{Gt(RRJ478)Byg} page

Mutation origin

• Mutant Cell Line: RRJ478
• Germline Transmission: Earliest citation of germline transmission: J:160964
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf
• Mutation details: A gene trap vector was inserted into intron 10, resulting in the production of a fusion protein encoded by the first 10 exons of MKL/myocardin-like 2 fused in-frame to a beta-galactosidase-neomycin phosphotransferase II reporter gene. Northern blot failed to detect transcript in mutants. A floxed splice acceptor site allows for recovery of transcription following cre-mediated recombination. (J:117226, J:160964)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRJ478	CC799059 ; (MGI Seq Detail)	5' RACE	Chr16:13218984-13219543 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  23 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	70 assay results
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mrtfb Mutation: 68 strains or lines available

References

• Original: J:160964 Li J, et al., Myocardin-related transcription factor B is required in cardiac neural crest for smooth muscle differentiation and cardiovascular development. Proc Natl Acad Sci U S A. 2005 Jun 21;102(25):8916-21
• All: 3 reference(s)