Sulf2^{Gt(PST111)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Sulf2^{Gt(PST111)Byg}
• Name: sulfatase 2; gene trap PST111, BayGenomics
• MGI ID: MGI:3587954
• Synonyms: Sulf2^{Gt(pGT1TMpfs)1Ucd}
• Gene: Sulf2 Location: Chr2:165915819-165997603 bp, - strand Genetic Position: Chr2, 86.31 cM, cytoband H3
• Alliance: Sulf2^{Gt(PST111)Byg} page

Mutation origin

• Mutant Cell Line: PST111
• Germline Transmission: Earliest citation of germline transmission: J:130163
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1TMpfs Vector Type: secretory trap
• Mutation details: A gene trap vector containing a beta-galactosidasse gene was inserted 1117 bp downstream of exon 3. This insertion creates a fusion between the first 138 amino terminus amino acids and beta-galactosidase. This allele is hypomorphic with 0.4% of wild type transcript detected at E14.5.
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  PST111	CC144663 ; (MGI Seq Detail)	5' RACE	Chr2:165958791-165958903 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  229 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	18 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sulf2 Mutation: 177 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:130163 Ratzka A, et al., Redundant function of the heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during skeletal development. Dev Dyn. 2008 Feb;237(2):339-53
• All: 5 reference(s)