Tln2^{Gt(RRI434)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Tln2^{Gt(RRI434)Byg}
• Name: talin 2; gene trap RRI434, BayGenomics
• MGI ID: MGI:3587955
• Synonyms: talin2ⁱⁿ¹²⁹⁵, Tln2^{Gt(pGT0Lxf)1Ucd}
• Gene: Tln2 Location: Chr9:67124369-67466985 bp, - strand Genetic Position: Chr9, 36.39 cM
• Alliance: Tln2^{Gt(RRI434)Byg} page

Mutation origin

• Mutant Cell Line: RRI434
• Germline Transmission: Earliest citation of germline transmission: J:102137
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: A gene trap vector containing beta-galactosidase and the engrailed 2 promoter integrated into intron 28 at amino acid 1295. The mutant allele was predicted to express endogenous amino acids 1-1295 fused to Beta-gal. Mutant tissues analyzed by Northern blot did not express transcript corresponding to the 3' UTR probe. LacZ signals were detected. Shorter transcripts were detected, possibly resulting from alternative splice forms. The testis splice form, which normally contains an extra 251 base pairs encoding a unique 30 amino acid N-terminus, only contained 204 residues of the wild-type sequence. The 3.5 kb kidney transcript begins downstream of the insertion site, and remained unaffected by the gene trap insertion. (J:102137)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRI434	CC326685 ; (MGI Seq Detail)	5' RACE	Chr9:67226601-67230426 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  73 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tln2 Mutation: 150 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:102137 Chen NT, et al., The N-terminal half of talin2 is sufficient for mouse development and survival. Biochem Biophys Res Commun. 2005 Nov 18;337(2):670-6
• All: 4 reference(s)