Cplaq14^CXB7/ByJ
QTL Variant Detail

Summary

• QTL variant: Cplaq14^CXB7/ByJ
• Name: circadian period of locomotor activity 14; CXB7/ByJ
• MGI ID: MGI:3588354
• QTL: Cplaq14 Location: unknown Genetic Position: Chr1, Syntenic

Variant origin

• Strain of Specimen: CXB7/ByJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers decreased circadian period locomotor activity compared to C57BL/6J and DBA/2J. (J:99678)
• Inheritance: Other (see notes)

Notes

Cplaq3 exhibits additive inheritance.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:99678

Linkage analysis was performed on 341 (BXD19/TyJ x CXB7/ByJ)F2 animals to confirm previously identified QTLs associated with circadian period as well as identify new loci. 85 informative genetic markers at an average spacing of 15 cM were used in the genome scan. It is estimated that 18% of the mouse genome was not covered in the analysis. Parental strain BXD19/TyJ is a recombinant inbred strain derived from C57BL/6J (B) and DBA/2J (D). BXD19/TyJ displays a 68 minute longer locomotor activity period and a 76 minute longer wheel running period compared to parental strain CXB7/ByJ, which is derived from BALB/cBy (C) and C57BL/6By (B). Several QTL accounting for 30% of the locomotor activity variance were mapped in this study.

A previously identified QTL on distal mouse Chromosome 1 named Cplaq3 was confirmed in this study.

03.11.2015 Curators Note: Because Cplaq3 was originally mapped in J:36675 in 1995 using 13 CXB (BALB/cBy x C57BL/6By) recombinant inbred strains, which differ from the recombinant inbred strains used here, we consider the currant study a separate mapping experiment and have named the QTL identified Cplaq14.

Cplaq14 mapped to 86.5 cM near D1Mit150 with LOD=5.66 between flanking markers D1Mit424 and D1Mit512 (Fig.3). This locus explains 10% of the phenotypic variance. BALB/cBy-derived alleles at Cplaq14, which in the present inter-RI F2 cross were inherited from CXB7, confer a shorter locomotor activity period with additive inheritance.

A suggestive locus for wheel running period mapped to 39.8 cM near D1Mit124 with LOD=3.18.

A previously identified QTL on distal mouse Chromosome 2 named Cplaq7 was confirmed in this study. Cplaq7 mapped to 74 cM near D2Mit224 with LOD=2.81 between flanking markers D2Mit482 and D2Mit286 (Fig. 3).

03.11.2015 Curator Note: Because Cplaq7 was originally mapped in J:29803 in 1995 using 26 BXD (C57BL/6J x DBA/2J) recombinant inbred strains, which also differs from the (BXD19 x CXB7) F2 cross used here, we consider this a separate map experiment and have named this QTL Cplaq15.

Significant linkage to circadian period locomotor activity mapped to 18.2 cM on mouse Chromosome 6 near D6Mit275 (LOD=2.84) between flanking markers D6Mit91 and D6Mit284 (Fig.3). This locus is named Cplaq9 (circadian period of locomotor activity 9). C57BL/6J-derived alleles at Cplaq9, which in the present inter-RI F2 cross were inherited from BXD19, confer shorter locomotor activity periods with dominant inheritance.

Significant linkage to circadian period locomotor activity mapped to 11 cM on mouse Chromosome 12 near D12Mit221 (LOD=5.2) between flanking marker D12Mit169 and D12Mit4 (Fig. 3). This locus is named Cplaq10 (circadian period of locomotor activity 10). BALB/cBy-derived alleles at Cplaq10, which in the present inter-RI F2 cross were inherited from CXB7, confer shorter locomotor activity periods with dominant inheritance. Cplaq10 maps near a previously identified free running period QTL named Frp3 (22 cM).

Significant linkage to circadian period locomotor activity mappedto 35 cM on mouse Chromosome 13 near D13Mit20 (LOD=3.77). This locus also shows linkage to wheel running period (LOD=2.8) and is named Cplaq11 (circadian period of locomotor activity 11). BALB/cBy-derived alleles at Cplaq11 which in the present inter-RI F2 cross were inherited from CXB7, confer shorter locomotor activity periods and shorter wheel running periods with dominant inheritance.

Significant linkage to circadian period locomotor activity mapped to 42 cM onChromosome 14 near D14Mit140 (LOD=4.33) between flanking markers D14Mit155 and D14Mit166 (Fig. 3).This locus is named Cplaq12 (circadian period of locomotor activity 12). BALB/cBy-derived alleles at Cplaq12, which in the present inter-RI F2 cross were inherited from CXB7, confer shorter locomotor activity periods with a dominant mode of inheritance.

Epistatic interaction between D4Mit155 (49.6 cM)on mouse Chromsome4 andD11Mit58 (65 cM) on mouse Chromosome 11 was detected.However, the effect of this locus pair did not reach statisticalsignificance.

References

• Original: J:99678 Hofstetter JR, et al., New quantitative trait loci for the genetic variance in circadian period of locomotor activity between inbred strains of mice. J Biol Rhythms. 2003 Dec;18(6):450-62
• All: 1 reference(s)